Possible reason:Samples are denatured insufficiently
Recommended solution:Ensure that the denaturation temperature of the hybridizer reaches the temperature required by the LBP’s manual, and the hospital needs to calibrate the equipment regularly
Possible reason:The probe is not sufficiently mixed before use
Recommended solution:Blow and beat the probe, mix well, and centrifuge shortly
Possible reason:The probe is not added
Recommended solution:Fully thaw the probe, to ensure that the pipette absorbs the reagent in the probe
Possible reason:The probe is expired
Recommended solution:Use the probe within the validity period
Possible reason:The amount of the probe is insufficient
Recommended solution:Ensure that the pipette sucks accurately, and increase the amount of the probe to 10 ul. Please do not dilute the probe
Possible reason:Bubbles are formed under the cover glass during hybridization
Recommended solution:The cover glass should cover the surface of the probe and tissues when placed , and should be squeezed gently to squeeze out bubbles
Possible reason:Hybridization conditions are unsuitable
Recommended solution:Set according to the requirements of LBP’s manual
Possible reason:The temperature of the hybridizer about digesting,washing and overnight are incorrect
Recommended solution:Ensure that the equipment such as the water bath meets the temperature required in LBP’s manual
Possible reason:Washing liquid or washing conditions are incorrect
Recommended solution:Set according to the requirements of LBP’s manual
Possible reason:Sample glass slides or probes are stored incorrectly
Recommended solution:Set according to the requirements of LBP’s manual
Possible reason:Counterstain is used incorrectly or expired
Recommended solution:Use DAPI within the validity period, set and use DAPI according to the requirements of LBP’s manual
Possible reason:The filter group selected during observation is inappropriate
Recommended solution:Recommended to use the parameter settings of the reagent filter required in LBP’s manual
Possible reason:The sample is not well fixed
Recommended solution:Try to replace with other paraffin blocks for treatment, or recommend pathologists to communicate with clinicians in time for fixation time and effect
Possible reason:The front and back of the glass slide are error
Recommended solution:Retreat, and pay attention to the front and back when the glass slide is taken out
Possible reason:Glass slides are dried insufficiently
Recommended solution:Before the probe drops the reagent onto the glass slide, guarantee that the ethanol solution on the glass slide has been fully volatilized
Possible reason:The film-sealing gum or cover glass are not removed
Recommended solution:Remove all film-sealing gum in time and uncover the cover glass with moderate strength
Possible reason:Proper film-sealing gum is not used
Recommended solution:Use special Fish Fixogum film-sealing gum. Do not use sealing film, transparent adhesive top, and preservative film
Possible reason:The service life of microscope mercury lamp is expired
Recommended solution:Please contact the microscope manufacturer to replace with a new mercury lamp
Possible reason:The microscope structure and objective lens are not appropriate for observing FISH samples, or the filter is oxidized
Recommended solution:Please contact the microscope manufacturer
